{"product_id":"therapeutic-peptides-methods-and-protocols-hardcover","title":"Therapeutic Peptides: Methods and Protocols - Hardcover","description":"\u003cdiv\u003e\u003cp style=\"text-align: right;\"\u003e\u003ca href=\"https:\/\/reportcopyrightinfringement.com\/\" target=\"_blank\" rel=\"nofollow\"\u003e\u003cb\u003eReport copyright infringement\u003c\/b\u003e\u003c\/a\u003e\u003c\/p\u003e\u003c\/div\u003e\u003cp\u003eby \u003cb\u003eAndrew E. Nixon\u003c\/b\u003e (Editor)\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eIn this volume we will cover technologies and associated methodologies that allow identification, characterization and application of therapeutic peptides.\u003c\/p\u003e \u003cp\u003e\u003c\/p\u003e \u003cp\u003eThe section covering identification will cover areas such as display on the surface of filamentous or lytic phage, substrate phage display, ribosome display and the use of peptide library pools. \u003c\/p\u003e \u003cp\u003e\u003c\/p\u003e \u003cp\u003eOften the output from library selection is not a peptide but the sequence of a peptide. We will therefore discuss approaches to produce peptide ligands both synthetically and through recombinant techniques. This section will also include a discussion of how peptides may be produced such that their serum residence time can be extended to allow weekly dosing such as fusions to other larger proteins or through modification with PEG.\u003c\/p\u003e \u003cp\u003e\u003c\/p\u003e \u003cp\u003eThe next section covers biophysical tools for characterization of peptide interactions with proteins. These include fluorescence polarization, equilibrium dialysis, radioligand binding assays, regular and array based SPR analysis.\u003c\/p\u003e \u003cp\u003e\u003c\/p\u003e \u003cp\u003eThe remaining section outlines methods for preparation and application of peptides as: imaging agents; targeting agents for radionuclei; receptor antagonists, and as tools to mediate cell penetration\u003c\/p\u003e\u003ch3\u003eBack Jacket\u003c\/h3\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eTherapeutic Peptides: Methods and Protocols\u003c\/i\u003e features biological methods for the preparation of peptide phage display libraries using both filamentous and lytic phage. With contributions from renowned authors in the field, the book also explores selection and screening of the prepared peptide libraries for peptides with the desired function and the subsequent characterization of the identified peptides. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003ePractical and dependable, \u003ci\u003eTherapeutic Peptides: Methods and Protocols\u003c\/i\u003e is an ideal guide for researchers from all backgrounds seeking methods for the identification of therapeutic peptide candidates.\u003c\/p\u003e\n            \u003cdiv\u003e\n\u003cstrong\u003eNumber of Pages:\u003c\/strong\u003e 279\u003c\/div\u003e\n            \u003cdiv\u003e\n\u003cstrong\u003eDimensions:\u003c\/strong\u003e 0.8 x 10.1 x 7.2 IN\u003c\/div\u003e\n            \u003cdiv\u003e\n\u003cstrong\u003eIllustrated:\u003c\/strong\u003e Yes\u003c\/div\u003e\n            \u003cdiv\u003e\n\u003cstrong\u003ePublication Date:\u003c\/strong\u003e October 22, 2013\u003c\/div\u003e\n            ","brand":"BooksCloud","offers":[{"title":"Default Title","offer_id":47704844566787,"sku":"9781627036726","price":212.38,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0588\/9310\/7359\/files\/c3hHb1RtTnYzSkhRMERybWYvSjA2dz09.webp?v=1770098015","url":"https:\/\/annizon.com\/en-it\/products\/therapeutic-peptides-methods-and-protocols-hardcover","provider":"annizon.com","version":"1.0","type":"link"}